Journal: Frontiers in Oncology
Article Title: TBX21 correlates with an immunosuppressive tumor microenvironment and Treg/Th17 imbalance in prostate cancer
doi: 10.3389/fonc.2025.1701148
Figure Lengend Snippet: TBX21 showed no influence on the immune checkpoint in CD4 + T and CD8 + T cells in 22RV1 xenograft nude mice model with human immune system. (A) Percentages of PD-1 + CD4 + T cells, Tim-3 + CD4 + T cells, and Lag-3 + CD4 + T cells were determined by flow cytometry. (B) Percentages of PD-1 + CD8 + T cells, Tim-3 + CD8 + T cells, and Lag-3 + CD8 + T cells were determined by flow cytometry. Flow-cytometry results are shown as representative gating plots together with scatter plots summarizing individual mice, with horizontal bars indicating mean ± SD. Statistical comparisons were performed using one-way ANOVA after arcsine transformation of proportional data; n = 6 mice per group.
Article Snippet: PD-1-FITC, Tim-3-PE, and Lag-3-PECy7 antibodies (CST, United States) were loaded into CD4 + T cells and CD8 + T cells, respectively.
Techniques: Flow Cytometry, Transformation Assay